Unraveling the regulatory dynamics of bidirectional promoters for modulating gene co-expression and metabolic flux in Saccharomyces cerevisiae.

ABSTRACT: Bidirectional promoters (BDPs) hold great promise for applications in synthetic biology by enabling co-expression of multiple genes with minimized promoter size. However, the lack of well-characterized BDPs along with an incomplete understanding of their regulatory mechanisms limits broader applications. Here, we conducted genome-wide screening and characterization of 749 BDP candidates containing a single shared nucleosome-depleted region in yeast Saccharomyces cerevisiae. A pronounced asymmetry in BDP strength was observed using both transcriptomic and fluorescence reporter analyses. We demonstrated that these unbalanced BDP strengths could be utilized for fine-tuning metabolic flux in yeast, achieving yields comparable to or exceeding those of commonly used constitutive or inducible pro

SUBMITTER: Jin Z 

PROVIDER: S-EPMC12159744 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature