Overexpression of Cks1 is associated with poor survival by inhibiting apoptosis in breast cancer.

ABSTRACT:

Purpose

The present study was undertaken to examine the amplification and expression status of Cks1 in breast cancer and its significance.

Methods

The amplification and expression status of Cks1 gene was examined by FISH, real-time PCR and immunohistochemistry, respectively. RNA interference was used to detect the effects of Cks1 on migration, invasion, cell cycle progress and apoptosis of breast cancer cells.

Results

Cks1 gene amplification was highly correlated with protein overexpression. Overexpression of Cks1 was strongly associated with lymph node metastasis and poor prognosis (P = 0.000, 95% CI (0.00-0.02); P = 0.008, 95% CI (0.001-0.05), respectively). Knockdown of Cks1 expression by RNA interference inhibited the cell cycle progress, migration and invasion ab

SUBMITTER: Wang XC 

PROVIDER: S-EPMC12160252 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature