Biochemical characterization of <i>Bifidobacterium bifidum</i> peptidoglycan d,l-endopeptidase BbMep that generates NOD2 ligands.

ABSTRACT: Soluble peptidoglycan fragments produced by the gut bacteria are key effectors in microbiota-host crosstalk. Here, we biochemically characterized BbMep, an NlpC/p60 domain-containing peptidoglycan d,l-endopeptidase from Bifidobacterium bifidum, which efficiently digests Lys- or Orn-type sacculi. Digestion of human stool-derived muropeptides by BbMep enhances NOD2 activation.

SUBMITTER: Kwan JMC 

PROVIDER: S-EPMC12160582 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature