Delineating Bovine Milk Derived Microvesicles from Exosomes Using Proteomics.

ABSTRACT: In the work presented herein, a simple serial-pelleting purification strategy combined with a mass spectrometry-based proteomics analysis was developed as a means of discerning differences in extracellular vesicle (EV) populations found in bovine milk samples. A sequence of ultracentrifugation speeds was used to generate changes in the abundances of EV populations, allowing for the identification of associated proteins. A metric was developed to determine the relative abundances of proteins in large EVs (>200 nm) and small EVs (<200 nm). Of the 476 proteins consistently found in this study, 340 are associated with vesicular components. Of these, 156 were heavily enriched in large EVs, 155 shared between large and small EVs, and 29 heavily enriched in small EVs. Additionally, out of 68 prot

SUBMITTER: Couse AD 

PROVIDER: S-EPMC12161284 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature