Investigation of the Antioxidant, Antibiofilm, and Endocrine-disrupting Potential of Amaranthus retroflexus Methanol Extract Used as Food: Network and Molecular Docking Analyses.

ABSTRACT: This study aims to investigate the antioxidant and iron chelating activities; phytochemical composition; and profile, antibiofilm activity, in silico molecular docking, and network analysis of the methanol extract of Amaranthus retroflexus. The extract showed an IC₅₀ value of 3.50 mg/mL in the DPPH assay and 6.61 mg/mL in the iron-chelating assay. The total phenolic and flavonoid contents were determined as 30.54 mg GAE/g DW and 29.28 mg QE/g DW, respectively. Antibiofilm tests revealed 7% inhibition against Pseudomonas aeruginosa, while biofilm formation increased by 10% in Staphylococcus aureus 25923. HPLC analysis identified key bioactive compounds, including ascorbic acid, gallic acid, vanillic acid, rosmarinic acid, oleuropein, rutin, and quercetin. Macroelement analysis indicated hig

SUBMITTER: Aytar M 

PROVIDER: S-EPMC12161443 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature