Identifying altered gene expression in neuroblastoma cells preceding apoptosis.

ABSTRACT:

Purpose

Concomitant differentiation and partial inhibition of proteasome trigger cell death in a neuroblastoma cell line (NBP2). Neither induction of differentiation nor partial inhibition of proteasome alone affects the viability of NBP2 cells. We wanted to identify genes whose expression alters under concomitant conditions and may account for cell death.

Methods

We used gel electrophoresis to analyze total genomic DNA for the detection of DNA fragmentation. Affymetrix Murine Genome U74A version 2 microarray was used to screen for approximately 6,000 functionally characterized genes and approximately 6,000 expressed sequence tags (ESTs). Real time PCR (RT-PCR) was performed to provide an accurate assessment of changes in gene expression.

Results

Concomitant different

SUBMITTER: Nahreini P 

PROVIDER: S-EPMC12161650 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature