YTHDF2-mediated m⁶A modification regulates mRNA stability of <i>Immediate early response gene 3</i> to modulate cell death in <i>Staphylococcus aureus-</i>induced bovine mastitis.

ABSTRACT:

Background

Staphylococcus aureus (S. aureus)-induced bovine mastitis is a major challenge for dairy production, causing significant economic losses. The regulatory mechanisms underlying host cell apoptosis and inflammation during S. aureus infection remain unclear. Therefore, this study investigates the role of N6-methyladenosine (m⁶A) modification and its reader protein YTHDF2 in regulating mRNA stability, apoptosis, and inflammation in bovine mammary epithelial cells (Mac-T cells) under S. aureus challenge.

Methods

MeRIP-seq, RIP-seq, and RT-qPCR were used to analyze m⁶A-modified IER3 mRNA and its interaction with YTHDF2. Apoptosis, necrosis, and mitochondrial function were assessed using YO-PRO-1/PI staining and JC-

SUBMITTER: Xing Y 

PROVIDER: S-EPMC12162568 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature