Adult-onset hypothyroidism induces granulosa cell apoptosis and affects ovarian follicle development in rats.

ABSTRACT:

Introduction

Hypothyroidism is a common endocrine disorder in women, which could lead to ovulation disorders and infertility, however, the effects of adult-onset hypothyroidism on ovarian development and gene expression characteristics need further study.

Methods

Here we conducted an adult-onset hypothyroidism rat model by using the methimazole (MMI) induction, then the hormone level changes and ovarian development were evaluated, furthermore, the effects of gene expression of granulosa cells and oocytes were detected by using single-cell RNA sequencing.

Results

Our results showed that, in addition to a decrease in thyroid hormones, the body weight was significantly reduced, while the estrus cycle was prolonged in the hypothyroidism group. Although the ovary/body weig

SUBMITTER: Li S 

PROVIDER: S-EPMC12163021 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature