Characterization of primordial germ cells from EG&K stage X chicken embryos.

ABSTRACT: Primordial germ cells (PGCs) derived from Eyal-Giladi and Kochav (EG&K) stage X embryos (XPGCs) represent the earliest germ cells in poultry. However, little is known about the XPGCs due the difficulty in the isolation and propagation of these cells. In this study, we report a method for the stable isolation and efficient in vitro propagation of XPGCs. We conducted a thorough analysis of the characteristics exhibited by XPGCs. First, we identified an average of 42 PGCs at this stage by using an DAZL-2a-mCherry genetically modified chickens. Three stable cell lines, one male and two female, were established from eight isolates using a filter paper ring and differential adhesion. Germ cell-related protein markers, including DAZL, CVH, and SSEA-1, were detected in the established XPGCs. Re-in

SUBMITTER: Chen D 

PROVIDER: S-EPMC12163151 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature