Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.

ABSTRACT:

Background

In contrast to many animal model studies, immunotherapeutic trials in humans suffering from cancer invariably result in a broad range of outcomes, from long-lasting remissions to no discernable effect.

Methods and findings

In order to study the T cell responses in patients undergoing a melanoma-associated peptide vaccine trial, we have developed a high-throughput method using arrays of peptide-major histocompatibility complexes (pMHC) together with antibodies against secreted factors. T cells were specifically immobilized and activated by binding to particular pMHCs. The antibodies, spotted together with the pMHC, specifically capture cytokines secreted by the T cells. This technique allows rapid, simultaneous isolation and multiparametric functional characterizat

SUBMITTER: Chen DS 

PROVIDER: S-EPMC1216330 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature