Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease <i>via</i> network pharmacology and <i>in vitro</i> experiments.

ABSTRACT:

Purpose

Metabolic dysfunction-associated steatosic liver disease (MASLD) poses a global health challenge with limited therapeutic options. Hesperetin, a flavonoid extracted from citrus, exhibits multiple pharmacological properties, but its mechanisms in MASLD-associated lipid metabolism remain unclear. This study aimed to explore the mechanism of hesperetin for treating MASLD.

Methods

Network pharmacology identified the therapeutic targets for MASLD. Key targets were selected based on network topology analysis. Subsequently, Kyoto Encyclopedia of genes and genomes (KEGG) pathways and gene ontology enrichment were conducted. Molecular docking was performed to evaluate the binding affinity between hesperetin and the identified key targets. In vitro validation included l

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12163577 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature