Round-Robin Comparison of RET Rearrangement Detection in ctDNA: A Novel Method for Limited Clinical Samples.

ABSTRACT:

Purpose

Next-generation sequencing assays for ctDNA analysis are routinely used in the care of patients with advanced non-small cell lung cancer. However, variable assay sensitivities in detection of fusions have been reported. Here, we report on the performance of detecting RET rearrangements in plasma across three commercial next-generation sequencing laboratories.

Experimental design

Banked plasma from the phase 3 LIBRETTO-431 trial was studied. For each patient (n = 60) with a known RET fusion by local tumor tissue genotyping, pretreatment plasma was divided into two 3-mL aliquots and tested on two of three: Guardant Health's Guardant360, Foundation Medicine's FoundationOneLiquid CDx, and Resolution Bioscience's ctDx-First. A round-robin comparison was performed across v

SUBMITTER: Paweletz CP 

PROVIDER: S-EPMC12163596 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature