Integrating Network Pharmacology and Experimental Validation to Elucidate the Mechanisms of Tang Shen Ping Decoction in Diabetic Kidney Disease.

ABSTRACT: Diabetic kidney disease (DKD) is a severe complication of diabetes, characterized by chronic inflammation and fibrosis. Tang Shen Ping Decoction (TSPD), a traditional Chinese medicine formulation, has shown therapeutic efficacy in DKD, yet its molecular mechanisms remain to be fully elucidated. To explore the multitarget mechanisms of TSPD, this study integrated network pharmacology, transcriptomic analysis, molecular docking, and molecular dynamics simulations, followed by in vivo and in vitro validation. A total of 248 active compounds and 649 potential targets of TSPD were identified, among which network pharmacology and transcriptomic integration highlighted 21 key genes involved in DKD pathogenesis. Protein-protein interaction network analysis further identified ALB, CCL2, EGF, FN1, a

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC12163699 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature