A mercury complex-based fluorescent sensor for biological thiols.

ABSTRACT: A novel fluorescent sensor, Hg(DST)₂, was developed for the selective detection of biological thiols, including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), in fully aqueous solutions at pH 7.2. The sensor exhibited significant fluorescence quenching upon coordination with Hg²⁺, which was reversibly restored in the presence of thiols due to the formation of thermodynamically favored Hg-thiol complexes. The OFF-ON fluorescence mechanism of the sensor was elucidated using DFT calculations. Fluorescence titration experiments revealed a strong linear correlation (R ² ≈ 0.998) between fluorescence intensity and thiol concentrations within the ranges of 0.34-8.00 μM for GSH, 0.47-10.00 μM for Cys, and 0.26-8.00 μM for Hcy, with corresponding

SUBMITTER: Hien NK 

PROVIDER: S-EPMC12163708 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature