Revised Diffusion Law Permits Quantitative Nanoscale Characterization of Membrane Organization.

ABSTRACT: The formation of functional nanoscopic domains is an inherent property of plasma membranes. Stimulated emission depletion combined with fluorescence correlation spectroscopy (STED-FCS) has been previously used to identify such domains; however, the information obtained by STED-FCS has been limited to the presence of such domains while crucial parameters have not been accessible, such as size (R_d), the fraction of occupied membrane surface (f), in-membrane lipid diffusion inside (D_in) and outside (D_out) the nanodomains as well as their self-diffusion (D_d). Here, we introduce a quantitative approach based on a revised interpretation of the diffusion law. By analyzing experimentally recorded STED-FCS diffusion law

SUBMITTER: Svobodova B 

PROVIDER: S-EPMC12163893 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature