Fusion with CTB enhances the immunogenicity and protective efficacy of MIC2 subunit vaccine against Eimeria tenella.

ABSTRACT: Coccidiosis is a parasitic disease caused by Eimeria species in chickens, leading to substantial threats to the poultry industry worldwide. Vaccination has emerged as a pivotal preventive strategy, with subunit vaccines gaining significant attention due to their safety. Microneme protein 2 (MIC2) of Eimeria tenella (E. tenella) is a promising candidate antigen for coccidiosis vaccination. However, its immune protection efficiency still needs to be further improved. Here, we report that MIC2, cholera toxin B subunit (CTB) and their fusion protein CTB-MIC2 are successfully recombinant expressed and produced in Pichia pastoris (P. pastoris). Notably, CTB and CTB-MIC2 exhibit high affinity for GM1 ganglioside, whereas MIC2 does not. Furthermore, upon administration to mice, the fusion protein

SUBMITTER: Wei H 

PROVIDER: S-EPMC12164184 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature