Histones in neutrophil extracellular traps (NETs) contain oxidative post-translational modifications induced by the myeloperoxidase oxidant hypochlorous acid.

ABSTRACT: Extracellular traps (NETs) released by neutrophils during inflammation play a role in clearing infection but also contribute to disease pathology. NETs consist of a DNA backbone containing histones, anti-microbial granule proteins, such as myeloperoxidase (MPO), and other proteins. MPO remains enzymatically active and generates hypochlorous acid (HOCl) to kill pathogens. However, HOCl also readily reacts with proteins, but whether histones and other NET proteins are modified by this oxidant is unknown. This is significant as post-translational modification of histones alters their intracellular and extracellular reactivity. In this study, we used a proteomic approach to characterise the protein composition of NETs and identify HOCl-induced oxidative modifications on histones and other prot

SUBMITTER: Hemmling H 

PROVIDER: S-EPMC12164213 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature