Metabolic abnormalities in the bone marrow cells of young offspring born to mothers with obesity.

ABSTRACT:

Background/objectives

Intrauterine metabolic reprogramming occurs in mothers with obesity during gestation, putting the offspring at high risk of developing obesity and associated metabolic disorders even before birth. We have generated a mouse model of maternal high-fat diet-induced obesity that recapitulates the metabolic changes seen in humans born to women with obesity.

Methods

Here, we profiled and compared the metabolic characteristics of bone marrow cells of newly weaned 3-week-old offspring of dams fed either a high-fat (Off-HFD) or a regular diet (Off-RD). We utilized a state-of-the-art flow cytometry, and targeted metabolomics approach coupled with a Seahorse metabolic analyzer.

Results

We revealed significant metabolic perturbation in the offspring of HFD-f

SUBMITTER: Phillips EA 

PROVIDER: S-EPMC12164757 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature