Conformational cycle and small-molecule inhibition mechanism of a plant ABCB transporter in lipid membranes.

ABSTRACT: In plants, ATP-binding cassette (ABC) transporters are crucial for nutrient uptake, phytohormone transport, and environmental response. It is of great interest to understand the mechanisms of these transporters and develop small-molecule modulators to regulate plant growth. Arabidopsis ABCB19 was recently shown to transport brassinosteroid, shaping hormone dynamics and plant architecture. However, the conformational cycle and inhibitor mechanism of ABCB transporters remain elusive. We reconstituted ABCB19 into lipid nanodiscs, where activity was drastically higher than in detergents, and determined its cryo-electron microscopy structures in substrate-free, substrate-bound, vanadate-trapped, and inhibitor-bound states. Inward-facing ABCB19 moved inward upon substrate binding and full

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12164952 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature