Ethylene-Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes.

ABSTRACT: Fruit of most apple varieties soften after harvest, and although the hormone ethylene is known to induce softening, the associated pathway is not well resolved. In this study, it is determined that MdEAEL1 (Ethylene-activated E3 ubiquitin Like 1) is specifically expressed during apple fruit postharvest storage, activated by ethylene, and interacts with the transcription factor MdZFP3 (zinc finger protein3). MdZFP3 is found to rely on an EAR (ethylene-responsive element binding factor-associated amphiphilic repression) motif to form a transcriptional repression complex with MdTPL4 (TOPLESS4)-MdHDA19 (histone deacetylase19), thereby downregulating the histone acetylation levels of the promoters of a range of cell wall degradation-related genes and inhibiting their transcription. MdEAEL1 ubiq

SUBMITTER: Li T 

PROVIDER: S-EPMC12165066 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature