M2a Macrophage-Dominant Microenvironment in Inflammation Attenuation and Wound Healing of Human Corneal Endothelial Cells.

ABSTRACT:

Purpose

To investigate the anti-inflammatory and wound-healing effects of an M2a macrophage-dominant microenvironment on human corneal endothelial cells (HCECs) in vitro.

Methods

Two in vitro corneal endothelial inflammation models were developed: a lipopolysaccharide (LPS)-induced inflammation-only model and a dual inflammation-and-wound model. HCECs were co-cultured with M2a macrophages or treated with M1 macrophage-derived exosomes (M1-exo), M2a macrophage-derived exosomes (M2a-exo), or epidermal growth factor (EGF)-preconditioned M2a-derived exosomes (EGF-M2a-exo). Gene and protein expression of inflammatory markers was assessed, and HCEC proliferation was evaluated using cell growth curves.

Results

Scratch wounding alone did not induce significant inflammation in

SUBMITTER: Lee SH 

PROVIDER: S-EPMC12165261 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature