Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER.

ABSTRACT: In situ hybridization (ISH) is a powerful tool for investigating the spatial arrangement of nucleic acid targets in fixed samples. ISH is typically visualized using fluorophores to allow high sensitivity and multiplexing or with colorimetric labels to facilitate covisualization with histopathological stains. Both approaches benefit from signal amplification, which makes target detection effective, rapid and compatible with a broad range of optical systems. Here, we introduce a unified technical platform, termed 'pSABER', for the amplification of ISH signals in cell and tissue systems. pSABER decorates the in situ target with concatemeric binding sites for a horseradish peroxidase-conjugated oligonucleotide, enabling the localized deposition of fluorescent or colorimetric substrates. We dem

SUBMITTER: Attar S 

PROVIDER: S-EPMC12165276 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature