Gαi3: a crucial biomarker and therapeutic target in bladder cancer.

ABSTRACT: This study investigates the role of Gαi3 in bladder cancer, focusing on its expression, functions, and mechanisms. Bioinformatics reveal that Gαi3 is overexpressed in bladder cancer, linked to poorer survival and higher tumor grades. Single-cell sequencing data confirm Gαi3 overexpression in bladder cancer epithelial cells. Additionally, Gαi3 is upregulated in tissues from locally treated patients and various bladder cancer cells. Silencing Gαi3 with targeted shRNA inhibited cell proliferation, migration, and invasion, activating caspase-mediated apoptosis. CRISPR/Cas9-mediated knockout of Gαi3 also exhibited significant anti-cancer effects, while overexpression enhanced cell proliferation and mobility. Gαi3 activates the Akt-mTOR pathway; silencing or knockout reduced phosphorylation of A

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12166043 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature