DExH-Box Helicase 9 Participates in De Novo Nrf2 Protein Translation Under Oxidative Stress.

ABSTRACT: Nrf2 transcript factor plays an important role in cellular defense against oxidative stress due to its control for expression of antioxidant and detoxification genes. We have found that Nrf2 gene undergoes de novo protein translation when mammalian cells encounter oxidative stress. Here, we report the discovery of DExH-box helicase-9 (DHX9), also known as RNA helicase A, as a binding protein for Nrf2 mRNA at 5'UTR. DHX9 binding to Nrf2 5'UTR increased with increasing doses (50-300 μM) of H₂O₂ or treatment time (10-120 min). This incease was in parallel with elevation of Nrf2 protein. Inhibiting DHX9 expression with siRNA or its activity with YK-4-279 inhibitor blocked H₂O₂ from inducing Nrf2 mRNA recruitment to the ribosomes or Nrf2 protein eleva

SUBMITTER: Dai W 

PROVIDER: S-EPMC12166428 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature