Proteomic analysis of hydrogen peroxide-treated human chondrocytes shows endoplasmic reticulum stress, cytoskeleton remodeling, and altered secretome composition.

ABSTRACT:

Background

Chondrocyte homeostasis is vital for maintaining the extracellular matrix (ECM) and overall cartilage health. In osteoarthritis (OA), for example, oxidative stress resulting from redox imbalances can disrupt chondrocyte homeostasis, leading to cartilage degradation. Hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), is a key mediator of oxidative stress and contributes to chondrocyte apoptosis and ECM degradation. Previous studies have explored individual protein responses to oxidative stress; however, a comprehensive proteomic analysis in chondrocytes has not been conducted. In this study, we aimed to assess the global proteomic alterations in chondrocytes exposed to H₂O₂ using a shotgun proteomics approach, which enab

SUBMITTER: Toledo TM 

PROVIDER: S-EPMC12166578 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature