Lung-resident memory Th2 cells regulate pulmonary cryptococcosis by inducing type-II granuloma formation.

ABSTRACT: Lung-resident memory T cells (lung TRMs) settle in the lung and respond rapidly to external antigens, and are therefore considered to have great potential for development of respiratory vaccines. Here, we demonstrate that lung-resident memory Th2 cells (lung TRM2) protect against pulmonary mycosis caused by Cryptococcus gattii. We developed novel whole-cell intranasal vaccines using a heat-inactivated C.gattii capsule-deficient strain cap59Δ, which induced ST-2⁺ Gata-3⁺ lung TRM2 specifically responding to C.gattii whole-cell antigen. Lung fungal burden and survival rate were significantly improved in immunized mice after infection challenge. The immunosuppressive agent FTY720 did not impact vaccine effectiveness, and adoptive transfer of lung TRMs into Rag-1-deficien

SUBMITTER: Ueno K 

PROVIDER: S-EPMC12167160 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature