Faecal amino acids stability: investigating optimal sampling conditions for analysis.

ABSTRACT:

Introduction

Faecal amino acids are promising non-invasive diagnostic biomarkers, but stability remains unclear.

Objectives

This study examined whether storage conditions, sampling site within the sample, freeze-thaw cycles, and the OMNImet^®·GUT device affect faecal amino acid concentrations.

Methods

Faecal samples from three donors underwent various pre-analytical conditions. Amino acids were analysed through targeted liquid chromatography-tandem mass spectrometry.

Results

Most amino acids remained stable across sampling sites and freeze-thaw cycles. Storage at -20 °C preserved integrity, whereas 4 °C and 20 °C storage led to variations. The OMNImet^®·GUT device stabilised some amino acids but showed inconsistencies.

Conclusion

Pre-

SUBMITTER: Opperman RCM 

PROVIDER: S-EPMC12167247 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature