Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation.

ABSTRACT:

Purpose

To characterize the hormonal environment in spent medium and cumulus cell gene expression during human IVM using oocytes from small antral follicles (SAFs) retrieved from surplus medulla tissue after ovarian tissue cryopreservation.

Methods

Immature oocytes from surplus medulla tissue underwent 42-h IVM in media with varying FSH and LH concentrations (0, 10, 100 IU/L FSH, and 100 IU/L FSH + 100 IU/L LH). Oocyte maturation was assessed by germinal vesicle (GV), metaphase I (MI), or metaphase II (MII) stages. Gene expression of FSHR, LHCGR, AMH, CYP19 A1, and INHA in cumulus cells was analyzed by RT-qPCR, and GDF9, AMH, inhibin-B, inhibin-A, and total inhibin were measured in the spent media by ELISA.

Results

Increased FSH concentrations downregulated FSHR expre

SUBMITTER: Cadenas J 

PROVIDER: S-EPMC12167398 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature