Acute dietary methionine restriction triggers cell cycle arrest and reversible growth defects in the neocortex.

ABSTRACT: Methionine is indispensable for cell proliferation, stem cell maintenance, and epigenetic regulation, three processes that are central to embryonic development. Here, we assessed the consequences of short-term dietary methionine restriction (MR) on mouse embryonic organ growth. In comparison with growth of the liver and heart which was unaffected, MR for 5 days led to a severe reduction in neuronal production and neocortex growth. Progenitor cohort labeling revealed a time-dependent sensitivity to MR and cell cycle analysis indicated that progenitors are stalled in S/G2 phases following MR. Unexpectedly, neuronal production was completely rescued at birth when switching the dam back to control diet, uncovering a mechanism of catch-up growth. We used imaging mass cytometry to probe metaboli

SUBMITTER: Saha S 

PROVIDER: S-EPMC12167773 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature