Dynamic relocation of PKA regulatory subunits during sperm capacitation: Linking PKA to the CatSper signaling complex.

ABSTRACT: To fertilize an oocyte, mammalian spermatozoa must undergo a maturation step known as capacitation that takes place after ejaculation. Protein kinase A (PKA) plays a fundamental role in capacitation in all mammalian species. Before capacitation, PKA is maintained in an inactive state where the catalytic subunits are bound to a dimer of inhibitory regulatory subunits. A key element in the regulation of PKA lies in its intracellular compartmentalization achieved by docking at A-kinase anchoring proteins (AKAP). Despite the crucial role of the modulation of local PKA activity in fertilization, its localization and mechanism of compartmentalization are not well understood. Here, we approach this problem using quantitative laser scanning microscopy and superresolution imaging to dissect the int

SUBMITTER: Novero AG 

PROVIDER: S-EPMC12167957 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature