Coordinated histone methylation loss and MYC activation promote translational capacity under amino acid restriction.

ABSTRACT:

Background

Cells adapt to nutrient fluctuations through both signaling and epigenetic mechanisms. While amino acid (AA) deprivation is known to suppress protein synthesis via mTORC1 inactivation, the epigenetic pathways that support cellular adaptation and recovery remain poorly understood. We investigated how chromatin and transcriptional changes contribute to maintaining translational capacity during AA restriction and priming cells for growth upon AA repletion.

Methods

Human cells were cultured under amino acid-replete or -depleted conditions, and global histone methylation levels were assessed by Western blotting and ChIP-seq. RNA-seq and chromatin-associated RNA-seq (chromRNA-seq) were used to evaluate gene expression and transcriptional output. Ribosome profiling and [

SUBMITTER: Cheng C 

PROVIDER: S-EPMC12168343 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature