Membrane-initiated estrogen receptor-α signaling in osteoblasts is crucial for normal regulation of the cortical bone in female mice.

ABSTRACT: Membrane-initiated estrogen receptor α (mERα) signaling has been shown to affect bone mass in murine models. However, it remains unknown which cell types mediate the mERα-dependent effects on bone. In this study, we generated a novel mouse model with a conditional C451A mutation in Esr1, which enables selective knockout of the palmitoylation site essential for the membrane localization of ERα (C451A^f/f). First, we used Runx2-Cre mice to generate Runx2-C451A^f/f mice with conditional inactivation of mERα signaling in Runx2-expressing osteoblast lineage cells. No significant changes were observed in body weight, weights of estrogen-responsive organs, or serum concentrations of estradiol between female Runx2-C451A^f/f and homozygous C451A^f/f littermat

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12170823 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature