Design of Epitopes from <i>Treponema pallidum</i> Lipoprotein Antigens for Syphilis Diagnosis and Treatment Prognosis.

ABSTRACT: Syphilis, a multistage sexually transmitted infection, causes severe complications if untreated. Accurate diagnosis remains difficult due to the low protein content of Treponema pallidum and in vitro culture difficulty. Advances in immunoproteomics have identified key antigens that enhance diagnostic accuracy. Epitopes of immunodominant antigen proteins Tp0171, Tp0435, Tp0574, Tp0684, and Tp0453 were designed by bioinformatics tools, and mimetic peptides were chemically synthesized. A diagnostic accuracy cross-sectional study was performed to validate a prototype technology as a serodiagnosis platform for syphilis. Five peptides were used as antigens in a peptide-based ELISA against serum samples from syphilis-positive or noninfected patients (n = 122). CETp0435 achiev

SUBMITTER: Borghezan LA 

PROVIDER: S-EPMC12172048 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature