Evaluating the predictive power of combined gene expression dynamics from single cells on antibiotic survival.

ABSTRACT: Heteroresistance and persistence are examples of mechanisms that can allow otherwise drug-susceptible bacteria to survive and resume growth after antibiotic exposure. These temporary forms of antibiotic tolerance can be caused by the upregulation of stress response genes or a decrease in cell growth rate. However, it is not clear how the expression of multiple genes contributes to tolerance phenotypes. Using fluorescent reporters for stress-related genes, we conducted real-time measurements of expression prior to, during, and after antibiotic exposure. We first identified relationships between growth rate and reporter levels based on auto- and cross-correlation analysis, revealing consistent patterns where changes in growth rate were anticorrelated with fluorescence following a delay. We t

SUBMITTER: Alnahhas RN 

PROVIDER: S-EPMC12172483 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature