Membrane transporter progressive ankylosis protein homologue (<i>ANKH</i>/<i>Ank</i>) partially mediates senescence-derived extracellular citrate and is regulated by DNA damage, inflammation, and ageing.

ABSTRACT:

Introduction

A considerable body of recent evidence supports citrate transport as a major regulator of organismal lifespan and healthspan. Citrate accumulates outside senescent cells in vitro and in vivo. However, the detailed mechanism of senescent cell extracellular citrate (EC) accumulation is not clear.

Methods

EC following various drug and cytokine treatments was measured in human fibroblast and keratinocyte conditioned medium by gas chromatography/mass spectroscopy and liquid chromatography/mass spectroscopy. Membrane transporters in similar human fibroblasts cultures were measured by western blotting and more extensively by reverse transcription and quantitative polymerase chain reaction (qPCR) in human fibroblasts, keratinocytes, myoblasts, adipocytes a

SUBMITTER: James EN 

PROVIDER: S-EPMC12174118 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature