RNA sequencing reveals circular RNA expression patterns in chronic intermittent hypoxia.

ABSTRACT: Circular RNAs (circRNAs) participate in diverse biological processes. However, whether circRNAs exhibit distinct expression patterns under obstructive sleep apnea (OSA)-induced chronic intermittent hypoxia (CIH) remains unexplored. We conducted RNA sequencing to compare expression profiles between CIH rats (n = 4) and normoxic controls (n = 4), identifying differentially expressed (DE) circRNAs. After filtering candidate circRNAs, we validated their expression in four OSA patients and four controls using qRT-PCR. Principal component analysis (PCA) was employed to confirm the diagnostic potential of these circRNAs. The miRanda software predicted target microRNAs (miRNAs), and the circRNA-miRNA regulatory network was visualized using Cytoscape software. In total, we identified

SUBMITTER: Li C 

PROVIDER: S-EPMC12174481 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature