Exploring the Anticancer Potential of Lamivudine-Loaded Polymeric Nanoparticles: <i>In Vitro</i> Cytotoxicity, Tissue Deposition, Biochemical Impact <i>In Vivo</i>, and Molecular Simulations Analysis.

ABSTRACT: Lamivudine is a synthetic nucleoside analogue to cytosine with a modified sugar moiety. It has potent action against Human Immunodeficiency Virus and chronic hepatitis. Recently, studies have also shown that lamivudine (3TC) can induce apoptosis in cancer cells and inhibit their proliferation, including breast cancer. We prepared polymeric nanoparticles using the double emulsification technique to incorporate polycaprolactone (PCL) as the polymer and lamivudine as the active compound. The nanoparticles were characterized by atomic force microscopy and dynamic light scattering. Then we carried out a full set of in vitro and in vivo analyses, including measurement of cytotoxicity, radiolabeling, biodistribution and biochemistry. The results showed the formation of 273 nm spheri

SUBMITTER: Gomes-da-Silva NC 

PROVIDER: S-EPMC12175167 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature