Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.

ABSTRACT:

Background

Reindeer (Rangifer tarandus tarandus) herding is based on access to seasonal pastures. Pastureland is, however, being lost and fragmented due to e.g. climate change, human activities, and predators, creating an increasing need for feeding and fencing. This alters disease occurrence, leading to a greater need for disease investigation tools. Knowledge of the activation of immune pathways during disease can be obtained by measuring cytokines, but no commercial methods are currently available for reindeer. This study investigated whether the MILLIPLEX® Bovine Cytokine Magnetic Bead assay could be used to detect interleukin (IL)-6, IL-8, IL-10, IL-17, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in reindeer cell supernatants and serum. Peripheral blood m

SUBMITTER: Frovoll TL 

PROVIDER: S-EPMC12175305 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature