Decoding hippocampal subfield and glial responses in ischemia using single-cell transcriptomics.

ABSTRACT:

Background

Stroke affects more than 12 million individuals worldwide annually, leading to lasting physical and cognitive impairments. The peri-infarct environment in the central nervous system, comprising glial and blood vessel cells, contributes to stroke progression. The hippocampal CA1 region is particularly vulnerable to ischemia, whereas the adjacent CA3-DG region exhibits different responses. Understanding the cellular and molecular alterations in these regions before and after ischemic insult can provide insights into stroke pathology and recovery mechanisms.

Methods

We conducted single-cell RNA sequencing on Sprague-Dawley rats subjected to four-vessel occlusion (4-VO) surgery, a model of transient global cerebral ischemia, and compared them with normal control rats.

SUBMITTER: Kwak D 

PROVIDER: S-EPMC12175379 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature