CHD7 regulates definitive endodermal and mesodermal development from human embryonic stem cells.

ABSTRACT:

Background

CHD7 encodes an ATP-dependent chromodomain helicase DNA binding protein; mutations in this gene lead to multiple developmental disorders, including CHARGE (Coloboma, Heart defects, Atresia of the choanae, Retardation of growth and development, Genital hypoplasia, and Ear anomalies) syndrome. How the mutations cause multiple defects remains largely unclear. Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as the thymus, liver, pancreas, and intestine.

Methods

In this study, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technique to delete the CHD7 gene in human embryonic stem cells (hESCs) to generate CHD7 homozygous mutant (CHD7^-/-), heterozygous mutant (CHD7^+/-

SUBMITTER: Hu R 

PROVIDER: S-EPMC12175391 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature