Analysis of the SARS-CoV-2 inactivation mechanism using violet-blue light (405 nm).

ABSTRACT: The study evaluated the effects of violet-blue light (VBL) on cell viability and replication, carbonylation of three structural proteins (S, E, and N) and one non-structural protein (NSP13), and direct damage to the RNA of SARS-CoV-2. The virus was exposed to increasing doses of VBL along with influenza A and B viruses to compare their susceptibility. At the highest dose (21.6 J/cm²), SARS-CoV-2 was significantly more susceptible to VBL than the influenza viruses, with a reduction in viral titer of 2.33 log₁₀. Viral RNA did not show significant changes after exposure to VBL, as demonstrated by next-generation sequencing and real-time PCR quantification, suggesting that the inactivation process does not involve direct nucleic acid damage. To exclude the role of the cul

SUBMITTER: Amodeo D 

PROVIDER: S-EPMC12175510 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature