Protection of LPS-induced intestinal injury in goslings by polysaccharide of Atractylodes macrocephala Koidz based on 16S rRNA and metabolomics analysis.

ABSTRACT: The protective effects of the polysaccharide of Atractylodes macrocephala Koidz (PAMK) against lipopolysaccharide (LPS)-induced intestinal injury in goslings was determined using 16S rRNA analysis of cecal contents and serum metabolomics analysis. PAMK was administered to goslings following LPS-induced intestinal injury, and its effects were assessed. PAMK significantly reduced the serum levels of inflammatory factors including interleukin (IL)-6 and C-reactive protein and decreased the expression of pro-inflammatory cytokines including interleukin(IL)-1[Formula: see text], IL-6, and toll-like receptor 2 in jejunal tissues. Moreover, PAMK significantly upregulated the relative mRNA expression levels of the tight junction proteins Zonula occludens-1, Occludin, Claudin, and Mucin-2, enhancin

SUBMITTER: Li W 

PROVIDER: S-EPMC12176298 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature