Identification of the microglia-associated signature in experimental autoimmune encephalomyelitis.

ABSTRACT:

Background

Multiple sclerosis (MS) is a chronic neuroinflammatory disorder characterized by demyelination and immune dysregulation, and microglia play a central role in disease progression. Despite this, the specific microglial gene signatures contributing to MS remain inadequately characterized.

Methods

We utilized an experimental autoimmune encephalomyelitis (EAE) mouse model and performed RNA sequencing to identify differentially expressed Messenger RNAs (DEmRNAs), Long Non-Coding RNAs (DElncRNAs), Circular RNAs (DEcircRNAs), and microRNAs (DEmiRNAs) in microglia. A machine learning approach incorporating five distinct algorithms was applied to select a robust multigene signature. The biological functions of the included genes were assessed through Gene Ontology (GO) and

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12176564 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature