Epigenetic editing at individual age-associated CpGs affects the genome-wide epigenetic aging landscape.

ABSTRACT: Aging is reflected by genome-wide DNA methylation changes, which form the basis of epigenetic clocks, but it is largely unclear how these epigenetic modifications are regulated and whether they directly affect the aging process. In this study, we performed epigenetic editing at age-associated CpG sites to explore the consequences of interfering with epigenetic clocks. CRISPR-guided editing targeted at individual age-related CpGs evoked genome-wide bystander effects, which were highly reproducible and enriched at other age-associated regions. 4C-sequencing at age-associated sites revealed increased interactions with bystander modifications and other age-related CpGs. Subsequently, we multiplexed epigenetic editing in human T cells and mesenchymal stromal cells at five genomic regions that b

SUBMITTER: Liesenfelder S 

PROVIDER: S-EPMC12176646 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature