Multigenerational cell tracking of DNA replication and heritable DNA damage.

ABSTRACT: Cell heterogeneity is a universal feature of life. Although biological processes affected by cell-to-cell variation are manifold, from developmental plasticity to tumour heterogeneity and differential drug responses, the sources of cell heterogeneity remain largely unclear^1,2. Mutational and epigenetic signatures from cancer (epi)genomics are powerful for deducing processes that shaped cancer genome evolution^3-5. However, retrospective analyses face difficulties in resolving how cellular heterogeneity emerges and is propagated to subsequent cell generations. Here, we used multigenerational single-cell tracking based on endogenously labelled proteins and custom-designed computational tools to elucidate how oncogenic perturbations induce sister cell asymmetry and phenot

SUBMITTER: Panagopoulos A 

PROVIDER: S-EPMC12176655 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature