<i>Brucella</i> infection induces chromatin restructuring in host cells to activate immune responses.

ABSTRACT:

Background

Brucella spp., facultative intracellular pathogens that cause brucellosis, drive pathogenesis by invading host cells and establishing intracellular persistence. While their molecular mechanisms are well-characterized, how Brucella induces chromatin restructuring in host cells remains poorly understood, representing a critical gap in host-pathogen interaction research.

Methods

Using an established in vitro infection model of Brucella-infected RAW264.7 murine macrophages, we integrated Hi-C, ATAC-seq, and RNA-seq to generate multi-omics datasets. Multidimensional comparative genomics approaches were employed to systematically map infection-induced changes in host chromatin architecture and functional genomic organization.

Results

O

SUBMITTER: Xie D 

PROVIDER: S-EPMC12176892 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature