Single-cell sequencing reveals dysregulated cell type perturbations and critical mediator communication remodelling in colorectal cancer.

ABSTRACT:

Background

The heterogeneity of colorectal cancer (CRC) and its complex immune microenvironment pose significant challenges for treatment. Understanding the cellular composition and dynamic changes is essential for uncovering mechanisms of tumour progression.

Methods

To investigate the cellular heterogeneity and immune microenvironment of CRC, identifying critical subpopulations, functional pathways, and prognostic biomarkers, single-cell transcriptomic data from 41 CRC samples across four datasets were integrated. Bioinformatic analyses identified cellular subpopulations, cell communication networks, and prognostic biomarkers. The expression patterns, clinical significance and biological function of TUBB were validated in vitro.

Results

A distinct epith

SUBMITTER: Xu C 

PROVIDER: S-EPMC12176898 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature