RFX3 is essential for the generation of functional human pancreatic islets from stem cells.

ABSTRACT:

Aims/hypothesis

The role of regulatory factor X 3 (RFX3) in human pancreatic islet development has not been explored. This study aims to investigate the function of RFX3 in human pancreatic islet development using human islet organoids derived from induced pluripotent stem cells (iPSCs), hypothesising that RFX3 regulates human islet cell differentiation.

Methods

We generated RFX3 knockout (RFX3 KO) iPSC lines using CRISPR/Cas9 and differentiated them into pancreatic islet organoids. Various techniques were employed to assess gene expression, cell markers, apoptosis, proliferation and glucose-stimulated insulin secretion. Single-cell RNA-seq datasets from human embryonic stem cell-derived pancreatic islet differentiation were re-analysed to investigate RFX3 expression in spec

SUBMITTER: Memon B 

PROVIDER: S-EPMC12176927 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature