Integrated spatial omics of metabolic reprogramming and the tumor microenvironment in pancreatic cancer.

ABSTRACT: Metabolic reprogramming is a defining feature of pancreatic cancer, influencing tumor progression and the tumor microenvironment. By integrating single-cell transcriptomics, spatial transcriptomics, and spatial metabolomics, this study visualized the spatial co-localization of metabolites and gene expression within tumor samples, uncovering metabolic heterogeneity and intercellular interactions. Spatial transcriptomics identified distinct pathological regions, which were further characterized using single-cell transcriptomic data and pathologist annotations. Pseudotime trajectory analysis revealed metabolic shifts along the malignant progression, while single-cell Metabolism (scMetabolism) delineated metabolic differences between pathological regions, classifying them as hypermetabolic or

SUBMITTER: Wu H 

PROVIDER: S-EPMC12177182 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature