The Fie1-PRC2 complex regulates H3K27me3 deposition to balance endosperm filling and development in cereals.

ABSTRACT: Polycomb repressive complex 2 (PRC2)-mediated H3K27me3 deposition is vital for cell fate determination during Arabidopsis thaliana endosperm development. Unlike the transient endosperm in Arabidopsis, cereals follow a different developmental program after the cellularization phase, producing a persistent endosperm. In contrast to the single, constitutively expressed Fertilization Independent Endosperm (FIE) gene in Arabidopsis, cereals have evolved a duplicated, grain-specific counterpart, such as maize ZmFie1 and rice OsFie1. However, their functions remain unclear. We applied Cleavage Under Targets and Tagmentation (CUT&Tag) to profile the dynamics of the H3K27me3 mark during maize endosperm development. We observed a genome-wide elevation of H3K27me3 levels at early stages, followed by

SUBMITTER: Wang J 

PROVIDER: S-EPMC12177492 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature